Investigation of relationship between hypericin contents and biological activity of aerial parts of hypericum perforatum L. From different regions of Iran

نویسندگانمریم اخباری,محسن ابراهیمیان
همایشچهارمین کنگره ملی گیاهان دارویی
تاریخ برگزاری همایش۲۰۱۵-۵-۱۲
محل برگزاری همایشتهران
سطح همایشملی
چکیده مقالهHypericin, a naphtodiantron with a beautiful red color, is a valuable medicinal compound which is found in different species of Hypericum genus, as the most important active compound. Via many medicinal properties of this valuable compound, reported in the literature, antimicrobial, antiviral, antidepressant, nonspecific kinas inhibition and dopamin β-hydroxilase inhibitoring could be reffered. Hypericum perforatumL. has been known as an important medicinal plant used in traditional Iranian medicin. In this research work, hypericin content of methanol extract from aerial parts of Hypericum perforatum from Kurdistan, Golestan and Qom province of Iran was evaluated and its relationship with antioxidant and antimicrobial activity of the extract was investigated. After removing chlorophyll via extraction using chloroform, methanol extract of the plant samples was isolated using soxhlet apparatus and hypericin contents were evaluated using UV-Vis spectrometric method. Antioxidant activity was evaluated using DPPH and β-carotene bleaching assays. Antimicrobial activity was studied using evaluation of inhibition zone diameter via disc diffusion method for 2 gram positive, 2 gram negative and one fungi strain. Hypericin content of the samples were in the order of: Kurdestan > Golestan > Qom; this order was also observed for antioxidant activity. About antimicrobial activity, for 5 studied microorganisms, as far as the number of sensitive microorganisms were considered, the order of Golestan>Kurdestan>Qom was exhibited; however the extract of the plant from Kurdestan has highest zone diameters for two staphylococcus species(S. aureus and S. epidermidis) and the observed activity for these bacteria has the same order of hypericin content and antioxidant activity.