Entrapment of Citrus limon var. pompia Essential Oil or Pure Citral in Liposomes Tailored as Mouthwash for the Treatment of Oral Cavity Diseases

AuthorsLucia Palmas,Matteo Aroffu,Giacomo L. Petretto,Elvira Escribano-Ferrer,Octavio Díez-Sales,Iris Usach,José-Esteban Peris,Francesca Marongiu,منصوره قوام,Sara Fais,Germano Orrù,Rita Abi Rached,Maria Letizia Manca,Maria Manconi
JournalPharmaceuticals
Page number1
Volume number13
IF5.863
Paper TypeFull Paper
Published At2020-08-28
Journal GradeScientific - research
Journal TypeElectronic
Journal CountryIran, Islamic Republic Of
Journal IndexSCOPUS ,PubMed ,JCR

Abstract

This work aimed at developing a mouthwash based on liposomes loading Citrus limon var. pompia essential oil or citral to treat oropharyngeal diseases. Vesicles were prepared by dispersing phosphatidylcholine and pompia essential oil or citral at increasing amounts (12, 25 and 50 mg/mL) in water. Transparent vesicle dispersions were obtained by direct sonication avoiding the use of organic solvents. Cryogenic transmission electron microscopy (cryo-TEM) confirmed the formation of unilamellar, spherical and regularly shaped vesicles. Essential oil and citral loaded liposomes were small in size (~110 and ~100 nm, respectively) and negatively charged. Liposomes, especially those loading citral, were highly stable as their physico-chemical properties did not change during storage. The formulations were highly biocompatible against keratinocytes, were able to counteract the damages induced in cells by using hydrogen peroxide, and able to increase the rate of skin repair. In addition, liposomes loading citral at higher concentrations inhibited the proliferation of cariogenic bacterium.

tags: Citrus limon essential oil; hydrogen peroxide; keratinocyte migration; scratch assay; anticaries activity; Streptococcus mutans