Effects of Irreversible Electroporation on Death of Triple Negative Breast Cancer Cells Under In Vitro Condition

Abstract

Background: Triple-negative breast cancer (TNBC) is a challenging subtype of breast cancer. Irreversible electroporation (IRE), a non-thermal method that uses high-voltage, low-energy electrical pulses to kill cells, has gained significant attention in recent years for its potential in cancer treatment. IRE offers the advantage of preserving the extracellular matrix, making it a promising approach for clinical studies. Methods: The MDA-MB-231 cell line was selected as a TNBC model. Two experimental groups and two control groups were established. Cell survival rates were assessed using the trypan blue staining (TBS) method in both control and experimental groups. Experimental groups were subjected to in vitro electroporation to determine optimal pulse parameters for destroying cancer cells without causing thermal damage. Results: After applying electrical pulses to the experimental groups, cell survival rates ranged from 19% to 58%, significantly lower than the control groups (89-94%). By analyzing these results, appropriate pulse parameters were identified to effectively destroy MDA-MB-231 cancer cells. Conclusion: IRE demonstrated the ability to destroy a significant portion of MDA-MB-231 cancer cells without causing thermal damage. This study highlights the potential of IRE as a promising treatment option for TNBC and other cancers.