Load Modeling of The Pulsed Power Generators for Electroporation Using Impedance Spectroscopy of Human Lung Normal and Cancer Cell

Abstract

In this paper, the load of the pulsed power generators is modeled using the impedance spectroscopy of human lung normal and cancer cells for
electroporation. Due to the differences in the electrical behavior of normal and cancer cells, cell modeling can be used in cancer diagnosis and treatment
selectivity (causing cell death in cancer tissues and not damaging the cells, and normal tissues) is effective. Also, due to the wide changes in cell
impedance under different conditions and the dependence of pulse generator structures on load impedance, cell modeling can have a significant effect
on the design of pulse generators and the creation of pulse parameters with the greatest effect. In this paper, the impedance of biological samples is
measured at different frequencies. Finally, for quantitative analysis of the electrical properties of cells, an accurate electrical equivalent circuit is
provided for the load. The proposed electrical model is not cell-focused but on the set of cuvette and its internal contents. This electrical model, taking
into account the effect of the two-layer electrical capacitor and parasitic effects, and expanding the frequency range from 100 Hz to 500 MHz. The
results show that the equivalent electrical circuit provided in most cases with an low error is consistent with the impedance measurements performed
for different samples