| Article Title En | Lipase immobilized on functionalized superparamagnetic few-layer graphene oxide as an efficient nanobiocatalyst for biodiesel production from chlorella vulgarise bio-oil |
|---|---|
| Authors | Tahereh Nematian, Alireza Shakeri, Zeinab Salehi, Ali Akbar Saboury |
| Journal | Biotechnology for Biofuels |
| Paper Type | Full Paper |
| Published At | 2020-3-20 |
| Journal Grade | ISI |
| Journal Type | Typographic |
| Journal Country | United Kingdom |
Abstract
Microalgae, due to its well-recognized advantages have gained renewed interest as potentially good feedstock for biodiesel. Production of fatty acid methyl esters (FAMEs) as a type of biodiesel was carried out from Chlorella vulgaris bio-oil. Biodiesel was produced in the presence of nano-biocatalysts composed of immobilized lipase on functionalized superparamagnetic few-layer graphene oxide via a transesterification reaction. A hybrid of few-layer graphene oxide and Fe3O4 (MGO) was prepared and characterized. The MGO was functionalized with 3-aminopropyl triethoxysilane (MGO–AP) as well as with a couple of AP and glutaraldehyde (MGO–AP–GA). The Rhizopus oryzae lipase (ROL) was immobilized on MGO and MGO–AP using electrostatic interactions as well as on MGO–AP–GA using covalent bonding. The supports, MGO, MGO–AP, and MGO–AP–GA, as well as nano-biocatalyst, ROL …