Biodiesel can be produced from animal fat or plant oil content. It is a safe, nontoxic, and free sulfur fuel with a higher flash point than gasoline. Macro- and microalgal biomass with rich triglyceride content is grown, harvested, and treated for the production of biodiesel. This biomass contains a higher oil content and grows faster than other potential plants for producing biodiesel. Oil extracted from algae is converted into fatty acid methyl esters (FAMEs) via a transesterification reaction. FAMEs, a well-known type of biodiesel, are used for transportation fuel. Different catalysts have been used to improve the selectivity and productivity of the process. Lipase, as a biocatalyst, makes the transesterification of triglycerides a highly selective process for FAME production. It has been demonstrated that enzyme immobilization on a suitable support can improve biocatalyst performance. Nanobiocatalysts for biodiesel production …