ESSENTIAL OIL COMPOSITION AND BIOLOGICAL ACTIVITIES OF EXTRACT OF ACINOS GRAVEOLENS FROM IRAN

Abstract

Determination of chemical constituents of the essential oil of Acinos graveolens and evaluation of biological activities of this plant methanol extract were performed in this study. Essential oil of A. graveolens was extract using simultaneous distillation extraction (SDE) method and have been analyzed by GC/FID and GC/Mass systems. Twenty-seven constituents representing 93.09% of the oil were identified both qualitatively and quantitatively. Germacrene-D (56.65%), bicyclogermacrene (8.69%) and caryophyllene oxide (7.46%) were detected as the plant major essential oil components. The plant extract was also tested for its antioxidant activities using 2,2- diphenyl-1-picrylhydrazyl (DPPH) and b-carotene/linoleic acid assays. Extract of A. graveolens showed a good activity in DPPH assay with IC50 value of (50.34±5.33 µg/ml) and a moderate activity in β-carotene-linoleic acid assay with inhibition percentage of 46.1±5.0%. Total phenolic compounds content of the extracts was recorded as 51.05±0.71 µg/mg. The plant also showed weak anticancer and antimicrobial activities in brine shrimp lethality and antimicrobial assays, respectively.