Conversion of bio-oil extracted from Chlorella vulgaris micro algae to biodiesel via Modified superparamagnetic nano-biocatalyst

چکیده مقاله

Rhizopus oryzae lipase (ROL) was loaded on Fe₃O₄ superparamagnetic nanoparticles (MNPs) with electrostatic attractions and covalent bonding. The nano-biocatalysts were used in biodiesel production process to investigate the effect of enzyme covalent bonding on catalytic performance. For nano-biocatalysts preparation, ROL was loaded on pure MNPs and MNPs functionalized with 3-aminopropyl triethylenesilane (MNP-AP), electrostatically. The enzyme was also bonded to MNPs functionalized with 3-aminopropyl triethylenesilane-glutaraldehyde (MNP-AP-GA), covalently. The hydrolytic activity, kinetic parameters, esterification productivity, and reusability of ROL/MNP, ROL/MNP-AP, and ROL/MNP-AP-GA as the four nano-biocatalysts were investigated and compared. The morphology, structure and magnetic characteristics of the MNPs were evaluated using FESEM, FTIR, XRD and VSM. The components of …