Amine and aldehyde functionalized mesoporous silica on magnetic nanoparticles for enhanced lipase immobilization, biodiesel production, and facile separation

چکیده مقاله

In this research, synthesis of fatty acid methyl ester (FAME) from olive oil as model feedstock was carried out in the presence of Rhizopus oryzae lipase (ROL) that was immobilized on the mesoporous silica magnetic nanoparticles (MNPs). MNPs were coated with mesoporous silica (MS) functionalized by amine and aldehyde groups used for immobilization of a lipase, used for biodiesel production, and easy magnetic separation of the immobilized lipase from the biodiesel product. MNPs were synthesized by using the co-precipitation method and coated with MS by sol-gel synthesis using cetyltrimethylammonium bromide (CTAB) as a template for mesoporous formation. ROL was immobilized on these supports via physical adsorption and covalent attachment. Enzyme loading efficiency on mesoporous was doubled compared to that on bare MNPs. Also, the covalent bonding led to an increase in enzyme loading …