Phylogenetic analysis of Trichodesma (Boraginaceae-Boraginoideae) based on ITS sequences data

Abstract

The rare and endangered plants of any country are important genetic resources that often require urgent conservation measures. Assessment of phylogenetic relationships and evaluation of genetic diversity is very important prior to implementation of conservation strategies for saving rare and endangered plant species. Boraginaceae is one of the largest family of the angiosperm and Trichodesma is one of the famous genus of this family. We used internal transcribed spacer sequences (ITS) of nuclear ribosomal DNA for the evaluation of sequence identity from the available Trichodesma species in the NCBI database. DNA sequences from the nuclear ITS region were exerted from 17 samples of the Trichodesma. The ITS sequences of 7 species from Caccinia were used as out-groups. Sequences were aligned using Bioedit Ver.7.0.9. The aligned data matrix was reconstructed by maximum parsimony (MP) and neighbor joining (NJ) employing MEGA Ver.7.0.26. We investigated the phylogeny relations between different species of Trichodesma. Also, Trichodesma was supported as non-monophyletic group.