ASSESSMENT OF CYTOTOXICITY OF (ASTRODAUCUS ORIENTALIS L.) DRUDE DIFFERENT PARTS USING BRINE SHRIMP (ARTEMIA SALINA) LETHALITY ASSAY

چکیده مقاله

Medicinal plantsconstitutea significant part of the flora and are extensively distributed in Iran.Therefore, identifying and expansion of information toward biological activity of these plants which can be appropriate foundation for the development of pharmaceuticals substances, is indispensable. In this direction, Astrodaucus orientalis (L.) Drude (Apiaceae Family) were selected and collected. A. orientalis (L.) Drude is regarded as a pleasant carrot-parsley flavor and so are used as a food additive or a salad vegetable in some parts of Iran and turkey. In the present work, methanol extract of whole of plant extracted by soxhlet apparatus were screened for its cytotoxicity using brine shrimp lethality test. The brine shrimp lethality assay is considered a useful tool for preliminary assessment of cytotoxicity. It has also been suggested for screening pharmacological activities in plant extracts. Total extract of leave and root-stem of A. orientalis (L.) Drude exhibited lethality percent 26% and 44.7% in 1000 of concentration, respectively. Thecurrent study with the support by brine shrimp bioassay showed theroot-stem methanolic extract of A. orientalis (L.) Drude have more anti canceral effects than own leaves [1].