| Authors | Morteza Yazdani, Fereshteh Jookar Kashi |
|---|---|
| Conference Title | 17 The International and Iranian Congress of Microbiology |
| Holding Date of Conference | ۲۰۱۶-۸-۲۳ |
| Event Place | Tehran |
| Presentation | SPEECH |
| Conference Level | International Conferences |
Abstract
Background and Aim:Tragopogon dubius is a species of salsify native to southern and central Europe and western Asia and found as far north and west as northern France. Methods:We collected this plant in april 2016 from south west of marivan. Extraction methanolic was performed with a Soxhlet apparatus. The extract was concentrated using a rotary evaporator. The antimicrobial activity of the extract was examined against a set of twelve microorganisms. The extract of Tragopogon dubius investigated for antimicrobial activity by agar well diffusion method. The dried plant ␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣ ␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣␣-Hilton agar (MHA) medium. The ␣␣␣␣␣␣ ␣␣␣ ␣␣␣ ␣␣␣ ␣␣␣␣␣␣␣␣␣␣ ␣␣␣␣␣␣␣␣␣␣␣␣ ␣␣␣␣␣ ␣␣␣ ␣␣␣ ␣␣␣ ␣␣␣␣ ␣␣␣␣␣␣␣␣ ␣␣␣␣␣␣␣␣␣ ␣␣␣␣␣ ␣␣␣␣␣␣␣␣␣ ␣DMSO (as negative control) and Gentamicin and rifampin (as positive controls) were placed on the inoculated agar (24 h at 37 °C). The diameters of inhibition zones were used as a measure of antimicrobial activity and each assay was repeated twice. Results:The extract of Tragopogon dubius showed activity against Staphylococcus epidermidis, Candida albicans and pseudomonas aeruginosa. The extract with inhibition zone diameter of 17,14 and 12 mm for Staphylococcus epidermidis, pseudomonas aeruginosa and Candida albicans , exhibited the best results respectively. Conclusion:Results of present study indicated that Tragopogon dubius extract had moderate antimicrobial activity.