Methanol Extract Of Mentha Piperita As Antimicrobial Agent

AuthorsMorteza Yazdani, Fereshteh Jookar Kashi
Conference Title18th International Iranian Congress of Microbiology
Holding Date of Conference۲۰۱۷-۸-۲۹
Event PlaceTehran
PresentationSPEECH
Conference LevelInternational Conferences

Abstract

Mentha piperita is a hybrid mint, a cross between water mint and spearmint. The plant is now widespread in cultivation in many regions of the world. Methods: We collected Mentha piperita in September 2016 from south west of Marivan. Extraction methanol was performed with a Soxhlet apparatus. The extract was concentrated using a rotary evaporator. The antimicrobial activity of the sample was examined against a set of twelve microorganisms. The microbial strains sensitive to the plant in disc diffusion assay were studied for their minimal inhibitory concentration (MIC) values using micro-well dilution assay method. Gentamicin and Rifampin were examined as positive controls and DMSO as negative control. Each assay was repeated twice. Results: The methanol extract of Mentha piperita (ME-MP) showed activity against S. epidermidis, C. albicans and S. aureus. The sample showed moderate to great antimicrobial activities with inhibition zone diameter from 12 to 23. The samples exhibited varied activity against the tested microorganisms with MIC ranging from 15.625 to 31.25 μg/ml. Conclusion: The results suggest that the extract seem to be alternative antibacterial agents to antibiotics and have the ability to overcome the bacterial resistance against antibiotics.