ANTIMICROBIAL ACTIVITY OF LEAF EXTRACT OF A NATIVE PLANT FROM SOUTHWEST OF MARIVAN

چکیده مقاله

Background and Aim:Tragopogon dubius is a species of salsify native to southern and central Europe and western Asia and found as far north and west as northern France. Methods:We collected this plant in april 2016 from southwest of marivan. Extraction methanolic was performed with a Soxhlet apparatus. The extract was concentrated using a rotary evaporator. To evaluate the antimicrobial activities extract of leaves were examined against a set of twelve microorganisms. The methanolic leaves extracts of Tragopogon dubius investigated for antimicrobial activity by agar well diffusion method. The dried plant extract was dissolved in DMSO (30 mg/ml) and filtered by 0.45 ml Millipore filters for sterilization. Suspension of bacteria (100 ll of 108 CFU/ml ) spread on müller-hinton agar (MHA) medium The discs (6 mm in diameter) impregnated with 10 ll of the extract solution (300 lg/disc)␣␣DMSO (as negative control) and Gentamicin and rifampin (as positive controls) were placed on the inoculated agar (24 h at 37 °C). The diameters of inhibition zones were used as a measure of antimicrobial activity and each assay was repeated twice. Results:The leaves extract of Tragopogon dubius showed activity against Staphylococcus epidermidis, Candida albicans and pseudomonas aeruginosa. Extract with inhibition zone diameter of 17,14 and 12 mm for Staphylococcus epidermidis (ethyl acetate fraction), pseudomonas aeruginosa (water fraction) and Candida albicans (hexan fraction), exhibited the best results respectively. Conclusion:Results of present study indicated that Tragopogon dubius leaf extract had moderate antimicrobial activity.