Potentiality of independent component regression in assessment of the peaks responsible for antioxidant activity of Thyme using gas chromatography-mass spectrometry technique

چکیده مقاله

Thyme species are medicinal herbs that are famous throughout the world and are used as antioxidant, antimicrobial, antispasmodic and antiseptic. The purpose of this study is identification of potential antioxidant compounds in the essential oils of Thyme. For this purpose, the obtained chromatogram and antioxidant activity of essential oils were used. The antioxidant activity of essential oils was measured by DPPH radical scavenging test. By coupling of chromatographic data and antioxidant activity assisted by independent component regression (ICR) can identify the peaks potentially responsible for antioxidant activity. The results ofthis technique were displayed as a regression graph, which peaks with negative regression coefficients cause antioxidant properties. Finally, GC–MS was used to indicate the structure of chemical components, which are responsible for antioxidant activity.