Identification of potential antioxidant compounds in Walnut (Juglansregia L.) leaves extract by linear multivariate calibration techniques and HPLC-DAD-MS

چکیده مقاله

The leaves of Juglans regia L. (Walnut) are used in traditional medicine in world due to its antifungal, antibacterial and antioxidant properties. In this study, the antioxidant activity in Walnut extracts was measured using 1,1-diphenyl-2-picrylhydrazyl (DDPH) assay and qualitative analysis was performed by HPLC-DAD-MS. The peaks potentially responsible for the antioxidant activity in Walnut samples were indicated by some linear multivariate calibration techniques with different preprocessing methods. From the studied techniques, orthogonal projection to latent structures (OPLS) was preferred to exhibit the potential antioxidant active compounds in Walnut leaves extract because of its high repeatability, simplicity and improved interpretability of the regression coefficients.