| Authors | سمیه چراغی,محمد طاهر,حسن کریمی مله,فاطمه کریمی,مهدی شبانی نوش آبادی,مرضیه علیزاده,Amani al-othman,Nevin Erk,Praveen KumarYegya Raman,CerenKaraman |
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| Journal | CHEMOSPHERE |
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| Page number | 132187 |
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| Volume number | 287 |
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| IF | ثبت نشده |
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| Paper Type | Full Paper |
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| Published At | 2021-09-07 |
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| Journal Grade | Scientific - research |
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| Journal Type | Electronic |
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| Journal Country | Iran, Islamic Republic Of |
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| Journal Index | SCOPUS ,JCR |
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Abstract
In this work, we report a novel enzymatic biosensor based on glutathione peroxidase (GSH-Px), graphene oxide
(GO) and nafion for the electrochemical sensing of glutathione (GSH) in body fluids. GSH-Px was immobilized
covalently via 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS) onto
modified glassy carbon electrode (GCE) decorated with GO and nafion and successfully used for sensing of GSH
in the presence of H2O2 as catalyst with Michaelis-Menten constant about 0.131 mmol/L. The active surface are
of GCE improve from 0.183 cm
2
to 0.225 cm
2
after modification with GO. The introduced biosensor (GSH-Px/
GO/nafion/GCE) was used for monitoring of GSH over the range 0.003–370.0 μM, with a detection limit of 1.5
nM using differential pulse voltammetric (DPV) method. The GSH-Px/GO/nafion/GCE was successfully applied
to the determination of GSH in real samples.
