Rapid and fast strategy for the determination of glutathione in the presence of vitamin B6in biological and pharmaceutical samples using a nanostructure based electrochemical sensor

Abstract

Cyclic voltammetry (CV), square wave voltammetry (SWV), electrochemical impedance spectroscopy (EIS) and double potential step chronoamperometry were used to investigate the electrochemical oxidation of glutathione (GSH) at a chemically modified electrode prepared by incorporating acetylferrocene (AF) and NiO nanoparticles (NiO/NPs) into a carbon paste matrix. For the mixture containing GSH and vitamin B6, the peak potentials were well separated from each other. Under optimal conditions in cyclic voltammetry, linear ranges spanned a GSH concentration from 0.2mM to 350.0mM and the detection limit was 0.09 mM at a signal-to-noise ratio of 3. The novel sensor was successfully used for the voltammetric determination of the GSH in real samples with satisfactory results.