Authors | Seyed Ahmad Dehdast, Hedayatollah Ghourchian, Hossain Ali Rafiee Pour |
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Journal | Journal of Paramedical Sciences |
Page number | 2008-2496 |
Serial number | 3 |
Volume number | 1 |
Paper Type | Full Paper |
Published At | 2010 |
Journal Grade | ISI |
Journal Type | Typographic |
Journal Country | Iran, Islamic Republic Of |
Abstract
A thin layer of poly methylen green (PMG) was covered on glassy carbon (GC) electrode surface by electrochemical polymerization method. In the next step by dropping a suspension of carboxylic acid functionalized carbon nano-tubes on the PMG/GC electrode a layer of CNTs was coated on the electrode. Thereafter, to immobilize the enzyme on electrode surface, three layers of PMG, alcohol dehydrogenase and PMG were added to the modified electrode, respectively. The Fourier transform infrared, scanning electron microscopy and cyclic voltammetry measurements clearly confirmed the successful immobilization of enzyme on the GC electrode.