Alcohol dehydrogenase immobilization on functionalized carbon nano-tubes modified electrode

AuthorsSeyed Ahmad Dehdast, Hedayatollah Ghourchian, Hossain Ali Rafiee Pour
JournalJournal of Paramedical Sciences
Page number2008-2496
Serial number3
Volume number1
Paper TypeFull Paper
Published At2010
Journal GradeISI
Journal TypeTypographic
Journal CountryIran, Islamic Republic Of

Abstract

A thin layer of poly methylen green (PMG) was covered on glassy carbon (GC) electrode surface by electrochemical polymerization method. In the next step by dropping a suspension of carboxylic acid functionalized carbon nano-tubes on the PMG/GC electrode a layer of CNTs was coated on the electrode. Thereafter, to immobilize the enzyme on electrode surface, three layers of PMG, alcohol dehydrogenase and PMG were added to the modified electrode, respectively. The Fourier transform infrared, scanning electron microscopy and cyclic voltammetry measurements clearly confirmed the successful immobilization of enzyme on the GC electrode.