| Authors | Somayeh Karimi, Hedayatollah Ghourchian, Parvaneh Rahimi, Hossain-Ali Rafiee-Pour |
|---|---|
| Journal | Analytical Methods |
| Page number | 3225–3231 |
| Volume number | 4 |
| Paper Type | Full Paper |
| Published At | 2012-8-01 |
| Journal Grade | ISI |
| Journal Type | Typographic |
| Journal Country | United Kingdom |
| Journal Index | ISI ,SCOPUS ,PubMed |
Abstract
An electrochemical biosensor was prepared by immobilizing cholesterol oxidase (ChOx) on a nanocomposite consisting of amine functionalized multi-walled carbon nanotubes and a room temperature ionic-liquid (1-butyl-3-methylimidazolium tetrafluoroborate). The biosensor was examined for cholesterol quantification in clinical serum samples. ChOx on the modified electrode exhibited a couple of quasi-reversible redox peaks corresponding to the direct electron transfer of FAD/ FADH2 buried in the enzyme structure. A formal potential of 400 mV was obtained for the immobilized enzyme in phosphate buffer solution (0.1 M, pH 7.4). Under the optimized experimental conditions, the biosensor exhibited a linear response towards cholesterol concentration from 26 nM to 3.4 mM, with a sensitivity of 540 mA mM 1and a detection limit of 12 nM.