| نویسندگان | Hossain-Ali Rafiee-Pour, Mohsen Behpour, Mahin Keshavarz |
|---|---|
| نشریه | Biosensors and Bioelectronics |
| شماره صفحات | 202–207 |
| شماره مجلد | 77 |
| نوع مقاله | Full Paper |
| تاریخ انتشار | 2016-1-01 |
| رتبه نشریه | ISI |
| نوع نشریه | چاپی |
| کشور محل چاپ | بریتانیا |
| نمایه نشریه | ISI ,SCOPUS ,PubMed |
چکیده مقاله
Small noncodingmicroRNAs(miRNAs)haveemergedasidealnoninvasivebiomarkersforearly-phase
cancer detection.Inthisreport,alabel-freeandsimpleelectrochemicalmiRNAbiosensorisdeveloped
based onemployingmethyleneblue(MB)asaredoxindicator.Thesuccessfullyimmobilizationofthe
single strandDNA(ss-DNA)probeandhybridizationwiththetargetmiRNAsequencewereconfirmed by
electrochemical impedancespectroscopy(EIS)andcyclicvoltammetry(CV)methods.Differentialpulse
voltammetry (DPV)techniquewasusedtorecordtheoxidationpeakcurrentofMBunderoptimal
condition andanincreaseinthepeakcurrentwasobservedafterhybridization.Byemployingthis
strategy,miRNAcandetectinarangefrom0.1to500.0pMwitharelativelylowdetectionlimitof
84.3 fM.TheelectrochemicalresponseofMBonss-DNAandduplexofmiRNA/DNAwascharacterizedby
CV andchronocoulometrymethod.Thelinearrelationbetweentheredoxpeakcurrents(Ip) andscanrate
(ν) indicatesthattheelectrontransfer(ET)betweenMBandtheelectrodesurfacewasmediatedbythe
miRNA/DNA π-stackedduplex.Thevalueofsurfacecoverage(Γ) wascalculatedthatindicatedincrease
amount ofMBonthesurfaceofmodified electrodeafterhybridizationeventandrevealedtheadsorption
of MBatmodified electrodeismonolayer.Also,theelectrontransferrateconstants(ks) ofMBwere
estimated. Theresultsofkineticanalysiswereconfirmed bychronocoulometrymethod.Thedis-
crimination abilityofmiRNAbiosensorevenagainstanoncomplementarytargetwasalsostudied.
Consequently,thisstrategywillbevaluableforsensitive,selectiveandlabel-freedetectionofmiRNA.