ISOLATION OF PROBIOTIC BACTERIA FROM HONEY AND EVALUATION OF THEIR PROBIOTIC PROPERTIES

چکیده مقاله

Background and Aim:Probiotics mean live microorganisms that have beneficial effects ontheir host’s health and can be isolated from many sources. Research show the use of probioticproducts are effective in eliminating harmful microbes. Due to the increasing expansion ofindustrial honey products instead of traditional honey, loss of many probiotic bacteria exist, soit is essential to isolate and identify the bacteria from traditional sources and used in themanufacture of industrial products. The aim of this study was to isolate the strains with highprobiotic potentiality which may exist in traditional honey. Methods:In this study, a total of 30 honey samples were collected from different parts of Iran.After gram stain and catalase test, biochemical and morphological properties were identified andapproved. The antimicrobial activity of probiotic bacteria on four bacteria were evaluated usingdisk diffusion method. Final molecular methods were employed to determine the strain types. Results:Three probiotic bacteria were isolated from thirty samples of honey using selective screening.Two of them are bacilli and the other is cocci. The antimicrobial activity of these bacteria wassignificantly. After investigation the probiotic properties of these isolates, they were identified by16S rRNA PCR. Conclusion:In the light of this study, it is observed that, natural honey is a source of potentialprobiotic strains. The results of this study showed that probiotics obtained from natural sourcesare good candidate for the prevention or control of disease. However, more in vivo studiesare necessary before they can be recommended for their beneficial effects in humans.