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Roohallah Nakhaei Sistani

Roohallah Nakhaei Sistani

Assistant Professor

College: Faculty of Chemistry

Department: Cell and Molecular Biology

Degree: Ph.D

Birth Year: 1979

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Roohallah Nakhaei Sistani

Assistant Professor Roohallah Nakhaei Sistani

College: Faculty of Chemistry - Department: Cell and Molecular Biology Degree: Ph.D | Birth Year: 1979 |

Assistant Professor

Molecular Genetics, University of Kashan, Kashan, Iran, 2013-Now

Field of interest: Biology of morphine addiction, miRNAs, Cancer biology, Herbal medicine, Bioinformatics. MicroRNAs are small RNA molecules which regulate many biological processes, including development, cancer, and also
addiction. And Bioinformatics, in my opinion, is a tool to explain many aspects of molecular biology, and unraveling neglected data, especially mass data. Iran has a great history of medicine based on herbs and working on herbs is promising for incurable diseases.

Ph.D

Molecular Genetics, Tarbiat Modares University, Tehran, Iran, 2008-2013.

During my Ph.D., I was working on microRNAs involving in morphine response in BE-2-C neuronal cell line as a model for molecular events happening in cells in the process of opioid addiction. Our data analysis indicates many biological pathways which could be involved in this process.

Master of Science

Molecular Genetics, Tarbiat Modares University, Tehran, Iran, 2002-2005.

Have you ever heard about DIAPOPS. This is a PCR-based technique which does not need for agarose gel electrophoresis. It uses a probe and enzymatic reaction for visualizing PCR products. I used this technique to detect fastidious adenoviruses in stool samples in my Ms.c.

Bachelor of Science

Plant Biology, Ferdowsi University, Mashhad, Iran, 1997-2002.

نمایش بیشتر

Investigation of serum cholestrol level in the individuals affected by obesity and correlation of it with rs2241766 and rs266729 polymorphisms of adipoq gene

Authorsنگار ربیعی فرادنبه،علی محمد احدی، روح الله نخعی سیستانی، راضیه بهارلو،
Conference TitleThe 4th confevence of protein and peptide sciernces (PPS4)
Holding Date of Conference2019-05-01 - 2019-05-02
Event Place1 - اصفهان
Presented byدانشگاه اصفهان
PresentationSPEECH
Conference LevelNational Conferences

Abstract

Background: Undoubtedly, obesity is one of the most common diseases in the community, with its side effects, such as metabolic aberration, liver and heart disease. So far, several studies have been conducted on the association of ADIPOQ gene polymorphisms with obesity and hypo-adiponctinemia in different population. Adiponectin is a protein hormone involved in regulating of fatty acid breakdown that is encoded by the ADIPOQ gene. Material and methods: In this study, blood samples were taken from the individuals registered to the TAMIN EJTEMAEE laboratory of Borujen city who were referred with obesity problems. LDL and free cholesterol level was analyzed on the blood of the patients using the El200 auto-analyzer. In the next step, Total DNA was extracted from the blood samples and subjected to RFLP and tetra-ARMS methods in order to investigation of rs2241766 and rs266729 polymorphisms of ADIPOQ gene respectively. Result and discussion: Our results confirmed the association of these polymorphisms at a significant level (p <0.05). The rs266729 polymorphism is located in the promoter region of ADIPOQ Gene. The presence of the G allele is likely to reduce the tendency of the transcription factors at -11377 position, which in turn can reduce the level of ADIPOQ expression and the related effects. Also, rs2241766 polymorphism showed a significant correlation with elevated cholesterol levels in the presence of T allele in this position from the exon 2 of ADIPOQ gene encoding adiponectin. In this case, a significant reduction in codon preference can reduce the expression of adiponectin protein. Our result can introduce two SNP marker applicable to the screening for high risk individuals for hypercholesterolemia.

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