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Roohallah Nakhaei Sistani

Roohallah Nakhaei Sistani

Assistant Professor

College: Faculty of Chemistry

Department: Cell and Molecular Biology

Degree: Ph.D

Birth Year: 1979

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Roohallah Nakhaei Sistani

Assistant Professor Roohallah Nakhaei Sistani

College: Faculty of Chemistry - Department: Cell and Molecular Biology Degree: Ph.D | Birth Year: 1979 |

Assistant Professor

Molecular Genetics, University of Kashan, Kashan, Iran, 2013-Now

Field of interest: Biology of morphine addiction, miRNAs, Cancer biology, Herbal medicine, Bioinformatics. MicroRNAs are small RNA molecules which regulate many biological processes, including development, cancer, and also
addiction. And Bioinformatics, in my opinion, is a tool to explain many aspects of molecular biology, and unraveling neglected data, especially mass data. Iran has a great history of medicine based on herbs and working on herbs is promising for incurable diseases.

Ph.D

Molecular Genetics, Tarbiat Modares University, Tehran, Iran, 2008-2013.

During my Ph.D., I was working on microRNAs involving in morphine response in BE-2-C neuronal cell line as a model for molecular events happening in cells in the process of opioid addiction. Our data analysis indicates many biological pathways which could be involved in this process.

Master of Science

Molecular Genetics, Tarbiat Modares University, Tehran, Iran, 2002-2005.

Have you ever heard about DIAPOPS. This is a PCR-based technique which does not need for agarose gel electrophoresis. It uses a probe and enzymatic reaction for visualizing PCR products. I used this technique to detect fastidious adenoviruses in stool samples in my Ms.c.

Bachelor of Science

Plant Biology, Ferdowsi University, Mashhad, Iran, 1997-2002.

نمایش بیشتر

Screening rs2241766 Polymorphism as a Susceptibility Index Parameter in Obese Patients with a High Level of Cholesterol

Authorsنگار ربیعی فرادنبه,روح اله نخعی سیستانی,علی محمد احدی
JournalJournal of Genetic Resources (J Genet Resour)
Page number198
Volume number8
IFثبت نشده
Paper TypeFull Paper
Published At2022-08-23
Journal GradeScientific - research
Journal TypeElectronic
Journal CountryIran, Islamic Republic Of
Journal IndexISC

Abstract

Obesity is a multifactorial disorder that has increased dramatically in recent years in developing countries. The disproportion of energy uptake and expenditure, which is the result of several factors such as diet, behavior, environment, as well as metabolic and genetic factors, could eventually lead to this disease. Mutations in genes that regulate appetite and metabolism could affect various aspects of obesity. Adiponectin protein, the product of the ADIPOQ gene, is an essential adipokine for controlling energy homeostasis and fat storage and is secreted in white adipose tissues. It is an insulin-sensitive hormone whose blood concentration declines in obesity. In this study, we examined the association of rs2241766 +45 T>G polymorphism of the ADIPOQ gene with obesity and blood levels of High-Density Lipoprotein (HDL), Low-Density Lipoprotein (LDL), Triglyceride (TG), and total cholesterol in the population of Borujen City. In this case-control study, blood samples were collected from people referred to Tamin Ejtemaee Laboratory of Borujen who suffered from obesity problems. Triglyceride, total cholesterol, LDL, and HDL levels were analyzed in the patient's blood using the alpha6 auto-analyzer. In the next step, total DNA was extracted from the blood samples and subjected to Restriction Fragment Length Polymorphism (RFLP) methods to investigate the rs2241766 polymorphism of the ADIPOQ gene. According to the results of this study, there is no significant correlation between any alleles and genotypes of this polymorphism with obesity (Chisquare p-value was 0.82 and 0.85 respectively for alleles and genotypes, oddsratio 1.155, 95% CI= 0.32-4.14). There is no significant correlation between the blood lipid characteristics of the patients and the genotypes of the studied polymorphism. According to our results, it seems that the alleles and genotypes of the ADIPOQ gene rs2241766 do not affect the etiology of obesity in our samples.