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Roohallah Nakhaei Sistani

Roohallah Nakhaei Sistani

Assistant Professor

College: Faculty of Chemistry

Department: Cell and Molecular Biology

Degree: Ph.D

Birth Year: 1979

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Roohallah Nakhaei Sistani

Assistant Professor Roohallah Nakhaei Sistani

College: Faculty of Chemistry - Department: Cell and Molecular Biology Degree: Ph.D | Birth Year: 1979 |

Assistant Professor

Molecular Genetics, University of Kashan, Kashan, Iran, 2013-Now

Field of interest: Biology of morphine addiction, miRNAs, Cancer biology, Herbal medicine, Bioinformatics. MicroRNAs are small RNA molecules which regulate many biological processes, including development, cancer, and also
addiction. And Bioinformatics, in my opinion, is a tool to explain many aspects of molecular biology, and unraveling neglected data, especially mass data. Iran has a great history of medicine based on herbs and working on herbs is promising for incurable diseases.

Ph.D

Molecular Genetics, Tarbiat Modares University, Tehran, Iran, 2008-2013.

During my Ph.D., I was working on microRNAs involving in morphine response in BE-2-C neuronal cell line as a model for molecular events happening in cells in the process of opioid addiction. Our data analysis indicates many biological pathways which could be involved in this process.

Master of Science

Molecular Genetics, Tarbiat Modares University, Tehran, Iran, 2002-2005.

Have you ever heard about DIAPOPS. This is a PCR-based technique which does not need for agarose gel electrophoresis. It uses a probe and enzymatic reaction for visualizing PCR products. I used this technique to detect fastidious adenoviruses in stool samples in my Ms.c.

Bachelor of Science

Plant Biology, Ferdowsi University, Mashhad, Iran, 1997-2002.

نمایش بیشتر

Identifying microRNAs relating to morphine response in BE (2)-C cell line by microRNA profiling

AuthorsRoohollah Nakhaei Sistani- Majid Sadeghizadeh- Bahram Mohammad Soltani
JournalProceedings of the National Academy of Sciences, India Section B: Biological Sciences
IF0.396
Paper TypeFull Paper
Published At2017
Journal GradeISI (WOS)
Journal TypeTypographic
Journal CountryIndia

Abstract

MicroRNAs (miRNAs) serve as critical modulators of post transcriptional gene regulation in response to stimuli such as narcotics. Hence, the analysis of miRNA expression may elucidate the molecular mechanisms underlying cellular response to morphine. Here, the
expression profile of 750 known miRNAs was generated in BE (2)-C neuroblastoma cell line by using the miRCURY LNA microRNA qPCR array system, in order to identify morphine responsive miRNAs. This population of miRNAs can be used potentially to provide an insight into the functional mechanisms of this drug. Bioinformatics analysis was used to predict the target genes of morphine responsive miRNAs, and the altered expression of selected targets was investigated through quantitative polymerase chain reaction. The result showed that a total of 10 miRNAs were up-regulated, while 33 miRNAs were downregulated in response to morphine treatment,  considering at least 1.75 fold change in expression between treated and untreated cells. Among them, hsa-miR-29a-5p, has-miR-
646, has-miR-1539, has-miR-412 and has-miR-937 showed the largest changes in expression level. Furthermore, the effect of multiple morphine responsive miRNAs on one target gene (cooperativity) was considered as the criterion to select target genes for the validation analysis. Cnr1, Jazf1, Nr2c2, Lonrf2, Bnc2, C1orf21 and eight more genes were selected based on cooperativity, and validated by using real time polymerase chain reaction.

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