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Roohallah Nakhaei Sistani

Roohallah Nakhaei Sistani

Assistant Professor

College: Faculty of Chemistry

Department: Cell and Molecular Biology

Degree: Ph.D

Birth Year: 1979

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Roohallah Nakhaei Sistani

Assistant Professor Roohallah Nakhaei Sistani

College: Faculty of Chemistry - Department: Cell and Molecular Biology Degree: Ph.D | Birth Year: 1979 |

Assistant Professor

Molecular Genetics, University of Kashan, Kashan, Iran, 2013-Now

Field of interest: Biology of morphine addiction, miRNAs, Cancer biology, Herbal medicine, Bioinformatics. MicroRNAs are small RNA molecules which regulate many biological processes, including development, cancer, and also
addiction. And Bioinformatics, in my opinion, is a tool to explain many aspects of molecular biology, and unraveling neglected data, especially mass data. Iran has a great history of medicine based on herbs and working on herbs is promising for incurable diseases.

Ph.D

Molecular Genetics, Tarbiat Modares University, Tehran, Iran, 2008-2013.

During my Ph.D., I was working on microRNAs involving in morphine response in BE-2-C neuronal cell line as a model for molecular events happening in cells in the process of opioid addiction. Our data analysis indicates many biological pathways which could be involved in this process.

Master of Science

Molecular Genetics, Tarbiat Modares University, Tehran, Iran, 2002-2005.

Have you ever heard about DIAPOPS. This is a PCR-based technique which does not need for agarose gel electrophoresis. It uses a probe and enzymatic reaction for visualizing PCR products. I used this technique to detect fastidious adenoviruses in stool samples in my Ms.c.

Bachelor of Science

Plant Biology, Ferdowsi University, Mashhad, Iran, 1997-2002.

نمایش بیشتر

Studies on combination of oxaliplatin and dendrosomal nanocurcumin on proliferation, apoptosis induction, and long non-coding RNA expression in ovarian cancer cells

AuthorsSeyed Hosseini E - Alizadeh Zarei M - Babashah S - Nakhaei Sistani R - Sadeghizadeh M - Haddad Kashani H - Amini Mahabadi J - Izadpanah F - Atlasi MA - Nikzad H
JournalCell Biology and Toxicology
IF3.390
Paper TypeOriginal Research
Published At2018/11/28
Journal GradeISI (WOS)
Journal TypeTypographic
Journal CountryNetherlands
Journal IndexISI, SCOPUS

Abstract

Abstract

Drug resistance remains a major challenge in the treatment of patients with ovarian cancer. Therefore, the development of new anticancer drugs is a clinical priority to develop more effective therapies. New approaches to improve clinical outcomes and limit the toxicity of anticancer drugs focus on chemoprevention. The aim of this study was to determine the effects of dendrosomal nanocurcumin (DNC) and oxaliplatin (Oxa) and their combination on cell death and apoptosis induction in human ovarian carcinoma cell lines analyzed by MTT assay and flow cytometry, respectively. The synergism effect of Oxa and DNC was analyzed using the equation derived from Chou and Talalay. In addition, real-time PCR was used to measure the effect of this combination on the expression levels of long non-coding RNAs with different expression in ovarian cancer and normal ovaries. Our data showed that the effect of DNC on cell death is more than curcumin alone in the same concentration. The greatest cell death effect was observed in combination of Oxa with DNC, while Oxa was added first, followed by DNC at 4 h interval (0/4 h). The findings indicated that DNC induced apoptosis significantly in both cell lines as compared to control groups; however, combination of both agents had no significant effect in apoptosis induction. In addition, combination of both agents significantly affects the relative expression of long non-coding RNAs investigated in the study as compared with mono therapy.

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