MicroRNAs Signature in IL-2-Induced CD4+ T Cells and Their Potential Targets

چکیده مقاله

MicroRNAs (miRNAs) are a class of small non-coding RNAs regulated gene expression at the post-transcriptional level. Many studies have investigated role of miRNAs in the biological processes such as proliferation, apoptosis, differentiation, and development. To evaluate role of miRNAs in proliferation and death of T cell, we performed miRNA profiling in activated CD4⁺ T cells after IL-2 induction and depletion. Proliferation rate of IL-2-induced cells was measured by MTT assay. Then quantitative RT-PCR arrays on 739 miRNAs revealed up- and down-regulation of 170 miRNAs in IL-2-induced CD4⁺ T cells relative to IL-2-depleted ones. In addition, in silico analysis predicted miRNA’s potential targets in pathways such as JAK/STAT and PI3K pathways. JAK1 expression, a potential target of modulated miRNAs, was decreased in IL-2-depleted cells. This study suggests that clonal expansion is regulated by miRNAs in the absence or presence of IL-2 by targeting genes implicated in JAK/STAT and PI3K pathways.