Loading and release study of ciprofloxacin from silica-coated magnetite modified by iron-based metal-organic framework (MOF) as a nonocarrier in targeted drug delivery system

چکیده مقاله

In this work, a magnetic and porous nanocomposite was prepared via synthesis of MIL-100(Fe) in the presence of magnetite nanoparticles that are well supported by silica (Fe3O4@SiO2). This nanocomposite due to its magnetic properties was used as an efficient nanocarrier for targeted ciprofloxacin (CIP) drug delivery. The loading and release behavior of CIP was evaluated in environments with different pH. The highest drug loading efficiency (DLE) was observed in AB (acetate buffer, pH:5.0) solutions and deionized water about 97.5% and 73%, respectively within 24 h. While during this period, the drug loading content (DLC) was estimated as 16.3% and 12.7%. The adsorption behavior of CIP was evaluated using Langmuir, Freundlich, Dubinin–Radushkevich (D–R), Temkin and Redlich-Peterson equations. The results revealed that the experimental data were fitted by Freundlich isotherm model. This isotherm shows that the drug is adsorbed multilayered on the nanocomposite active sites. Also the adsorption process was completed by following pseudo-second-order and intra-particle adsorption kinetics in 48 and 30 h under physiological and acidic conditions, respectively. From the analysis of BET and EDX-map results, it was revealed that the drug is both loaded into the pores of the nanocomposite and is uniformly absorbed on the surface of the nanocomposite. The kinetics of drug release was also investigated. Among the perused release kinetic mechanisms, the Korsmeier-Peppas model is inconsistent with the results well. Antibiogram analysis was performed to determine the zone of inhibition of Pseudomonas aeruginosa and Staphylococcus epidermidis. The drug-loaded nanocarrier was evaluated for in vitro cytotoxicity using the MTT assay at different times and concentrations in MCF-7 cancer and L929 normal cell lines. The results showed significant biotoxicity of the drug-loaded nanocarrier against cancer cell lines and less toxicity on normal cells. Due to the magnetic property of the nanocarrier, it has potential as an engaged nanocarrier for future in vivo targeted drug delivery studies.