Protease enzyme applications and ways to improve its performance

Abstract

Protease enzymes can hydrolyze peptide bonds. Proteases are widely used in commercial, medical and research processes. Proteases make up 60% of the total market of industrial enzymes. Several proteases of animal origin, such as pepsin, trypsin and proteases of plant origin, such as papain, were discovered and characterized in the early 1800s or the early 1900s, and later microbial proteases were also considered and due to several advantages associated with them quickly became popular. Increasing protease efficiency is critical for various applications, including industrial processes, food production, and biomedicine. Recent research has focused on several strategies to optimize protease performance, including immobilization techniques, engineering methods, and cold-adapted enzymes.