Analysis of Levodopa in the Presence of Vitamin B6 Using Carbon Paste Electrode Modified with 1-Butyl-3 methylimidazolium Hexafluorophosphate and CuO Nanoparticles

Abstract

A carbon paste electrode modified with 1-butyl- 3-methylimidazolium hexafluorophosphate ionic liquid (BMIPF6) and CuO nanoparticles (CuO/NPs) (CPE/ BMIPF6/CuO/NPs) was fabricated and used for square wave voltammetric analysis of levodopa in the presence of vitamin B6. The elemental analysis, SEM and XRD methods were used for characterization of synthesized CuO nanoparticle. CPE/BMIPF6/CuO/NPs exhibited high electrical conductivity toward the electro-oxidation of levodopa at a pH=7.0 as best experimental condition. Using CPE/ BMIPF6/CuO/NPs the levodopa and vitamin B6 peaks are separated and oxidized at potentials of 0.565 V and 0.835 V, respectively; hence levodopa can be detected in the presence of vitamin B6. The electrochemical response shows a linear relationship from concentration of levodopa and vitamin B6 in the ranges of 0.06-1000 mM and 0.1- 700.0 mM, respectively. Finally, CPE/BMIPF6/CuO/NPs were applied as high performance tool for determination of levodopa and vitamin B6 in real samples.