Evaluationof antimicrobial activity ofsome۱,۴-dihydropyridine derivatives containing nitro groups

چکیده مقاله

1,4-dihydropyridines are multifunctional molecules which exhibit various pharmacological activities such as antihypertensive, antianginal, antitumor, anti-inflammatory, antimicrobial activity [1,2]. In this study, three derivatives of 1,4-dihydropyridine such as 3,5-diethoxy carbonyl-4- phenyl-2,6-dimethyl-1,4-dihydropyridine, 3,5-diethoxy carbonyl-4-(4-nitrophenyl)-2,6-dimethyl-1,4-dihydropyridine and 3,5-diethoxy carbonyl-4-(3-nitrophenyl)-2,6-dimethyl-1,4-dihydropyridine were synthesized and investigatedfor antimicrobial activity. Following microbial strains were provided by Iranian Research Organization and Technology and used in this research: Pseudomonas aeruginosa, Escherichia coli, Klebsiella pneumonia, Shigelladysenteriae, Proteus vulgaris and Salmonella paratyphi-A serotype as examples of Gram negative bacteria, Bacillus subtilis, Staphylococcus aureus and Staphylococcus epidermidis as examples of Gram positive bacteria, Candida albicans, Aspergillusniger and Apergillusbrasilliensis as examples of fungal strains. Agar diffusion technique was used for the assesment of preliminary antibacterial and antifungal activities [3]. The minimum inhibitory concentrations (MIC) for the compounds which showed growth inhibition zones, were estimated by micro-well dilution assay method [4]. Our results indicated that 3,5-diethoxy carbonyl-4-(4-nitrophenyl)-2,6-dimethyl-1,4-dihydropyridine and 3,5-diethoxy carbonyl-4-(3-nitrophenyl)-2,6-dimethyl-1,4-dihydropyridine were active against Gram positive bacteria while 3,5-diethoxy carbonyl-4- phenyl-2,6-dimethyl-1,4-dihydropyridine was only active against Bacillus subtilis. The MIC results showed that 3,5-diethoxy carbonyl-4-(4-nitrophenyl)-2,6-dimethyl-1,4-dihydropyridine was more effective against Gram positive bacteria as compared with 3,5-diethoxy carbonyl-4-(3-nitrophenyl)-2,6-dimethyl-1,4-dihydropyridine.