Multivariate curve resolution-alternating least squares assisted voltammetry for simultaneous determination of non-steroidal anti-inflammatory drugs

چکیده مقاله

In the present work differential pulse voltammetry (DPV) coupled with multivariate curve resolution-alternating least squares (MCRALS) was applied for simultaneous determination of two non-steroidal anti-inflammatory drugs (mefenamic acid (MEF) and diclofenac (DCF) at the surface of a Cu(OH)2 nanoparticles modified carbon paste electrode (Cu(OH)2 nanoparticles/CPE). Characterization of the synthesized Cu(OH)2 nanoparticles was carried by scanning electron microscopy (SEM). Multivariate optimization based on central composite rotatable design (CCRD) coupled with response surface methodology (RSM) was used to optimize all effective experimental variables on DPV responses of the two drugs. Multivariate curve resolution alternating least squares (MCR-ALS) was applied for the resolution and quantification of MEF and DCF with a high degree of overlapping voltammograms. The lack of fit (LoF) of 3.74% was obtained by MCR-ALS. The proposed method was successfully applied for simultaneous determination of two drugs in human serum samples.