Potentiality of chemometric approaches for the determination of(+)-catechin in green tea leaves at the surface of multiwalled carbonnanotube paste electrode

چکیده مقاله

Differential pulse voltammetry technique assisted by chemometric methods such as multivariate curveresolution-alternating least squares (MCR-ALS) has been proposed as a valuable approach for (+)-catechindetermination in the presence of gallic acid at the surface of multiwalled carbon nanotube paste elec-trode. Central composite design and response surface methodology were used to optimize the influencingparameters. To determine (+)-catechin in the presence of unexpected electroactive interference with avery high degree of overlapping, second-order electrochemical data were generated by changing thepulse height as an instrumental parameter. After potential shift correction, MCR-ALS results show thatsecond-order calibration could be applied with great success for electroanalytical determination of highlyoverlapped electroactive species. The linear least-squares calibration curve based on the area under con-centration profile was provided over the range of 0.10–2.69 M for (+)-catechin, whereas detection limitwas found to be 0.017 M. Also in this study, the effect of rotational ambiguity associated with a particularMCR solution under a set of constraints was investigated.