Cytotoxicity effects of Carnosine peptide analogues on mitochondria Isolated from human Glioblastoma Multiforme

چکیده مقاله

Glioblastoma is the most malignant primary tumor of the central nervous system in adults. Glioblastoma is still often abbreviated “GBM” is the highest grade glioma tumor, is the most malignant form of astrocytoma, and is synonymous with a grade IV glioma. Astrocytomas are tumors that arise from astrocytes (star-shaped cells) that make up the “glue-like” or supportive tissue of the brain. The present study evaluates the effect of the naturally occurring linear and cyclic Carnosine analogues on primary cell cultures established from mitochondria Isolated from human glioblastoma multiforme (GBM).Mitochondria human glioblastoma multiforme and evaluation of the selective cytotoxic effect of dipeptide Carnosineanalogues was isolated and cellular parameters related to apoptosis signaling were then determined. Our results showed that high toxicity synthesized linear and cyclic Carnosine analogues with 10μg/mL concentration in MTT assay, a raise in mitochondrial reactive oxygen species (ROS) level, after exposure of mitochondria only isolated from the human glioblastoma multiforme. Based on the overall results, cyclic Carnosine analogues against linear Carnosine analogues would be encouraging to develop new anticancer agents and may be considered as a promising complementary therapeutic agents for the treatment of glioblastoma. The synthesized Carnosine analogues were characterized by using different methods such as, LC-MS.