Synthesis, cytotoxic agents of Carnosine peptide analogues on mitochondria obtained from the liver of HCC rats

چکیده مقاله

In this study, HCC was induced by diethylnitrosamine (DEN), as an initiator, and 2- acetylaminofluorene (2-AAF), as a promoter. Rat liver hepatocytes and mitochondria for evaluation of the selective cytotoxic effect of Carnosine dipeptide analogues was isolated and cellular parameters related to apoptosis signaling were then determined. Our results showed that high toxicity synthesized linear and cyclic Carnosine analogues with concentration 10 μg/mL in MTT assay, a raise in mitochondrial reactive oxygen species (ROS) level, after exposure of mitochondria only isolated from the Hepatocellular carcinoma (HCC group). Based on the overall results, cyclic Carnosine analogues against linear Carnosine analogues would be encouraging to develop new anticancer agents and may be considered as a promising complementary therapeutic agents for the treatment of HCC. The synthesized Carnosine analogues were characterized by using different methods such as, LC-MS.